reach out to MicroscopeMaster because they, as hobbyists, would like to buy
their first microscope in the near future but are unsure of which to buy. They
don’t understand enough about microscopes and the choices available as there is
a wide range to choose from.
Customers may understand the types of images that are produced through the various imaging techniques such as phase contrast, epi-fluorescence and DIC but are wondering about the ease of conversion from one technique to the next through the use of filters and accessories.
at MicroscopeMaster we want to emphasize that choosing a microscope depends on
what you are aiming to observe. For instance, rocks,
gems and opaque materials require an epi-illumination microscope. In contrast, bright
field microscopy is that standard for viewing most biological materials so that
would include DIC, phase contrast and fluorescence. Considering this,
microscopes are commonly classified as "biological" or
For a person wanting
to observe semi-micro specimens, be it biological (insects, larvae, fish,
tissues, pond critters, plant cells, outlines of bacteria etc.) or inorganic
(gems, coins, animal skeletons and shells, computer chips and electronic parts,
etc.), a stereo microscope would be a great alternative.
observation is low magnification and is usually done without any previous
preparation directly on the just acquired specimen, in contrast to a compound
light microscope.The same stereo microscope
may be used with most kinds of illumination: epi, lateral (oblique),
transillumination, fluorescence and darkfield.
You do need a
transmitted light (biological) microscope for most biological specimen. Specifically, light does not transmit
through a rock or electronic part.
Moreover, the location of the light depends on the subject.
Keep in mind that buying
your first microscope, in the course of your hobby, does not have to be a long
drawn out decision. You can purchase
a good, serviceable biological compound microscope for around $200.
A good used
microscope off of Ebay for example will normally sell for what you initially paid
The biggest part of
your microscopy hobby is specimen preparation. This requires the means to prepare a specimen, like cutting
microscopically thin slices and using stains etc. Before you go on to more advanced techniques in microscopy
like phase contrast and fluorescence, you will want to have sufficient practice
in the basic techniques.
A MicroscopeMaster reader wrote:
“I taught a high school biology class last year. We had students
make histograms of how many onion cells were in different stages of
mitosis. We could see the cells dividing and the chromosomes aligned in
the median plate all for microscopes most people on the list would call
"total cheap junk". My $180 used American Optical scope was in
a class above what the school lab used but the school lab scopes did the job."
If you are unsure about shopping from Ebay, then be sure to check out the Amscope and the Omax brands of microscopes.
Great for beginners, well worth the money, sturdy and reliable. Available at Amazon.com.
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The material on this page is not medical advice and is not to be used for diagnosis or treatment. Although care has been taken when preparing this page, its accuracy cannot be guaranteed. Scientific understanding changes over time.
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