Cheek Cells Under the Microscope
Requirements, Preparation and Staining
Cheek cells are eukaryotic
cells (cells that contain a nucleus and other organelles within enclosed in a
membrane) that are easily shed from the mouth lining. It is therefore easy to
obtain them for observation.
Some of the main parts of a
The cell membrane (outer boundary of the cell)
Cytoplasm (the fluid within the cell)
A nucleus (at the center of the cell and controls cell functions)
Organelles (e.g. mitochondria-Organelles are cell structures with
Using biological stains
such as methylene blue, it is possible to clearly observe and differentiate the
different parts of a cell. This is because the stain will color some parts of
the cell and not others, allowing them to be clearly observed.
- Sterile cotton swab
- Clean, sterile microscope slides
- Microscope cover slips
- Methylene Blue solution (0.5% to 1%)
- A dropper
- Blotting paper/Tissue paper
How to Prepare a Wet Mount of Cheek Cells
Before starting, it is
always important to ensure that the working surface is clean and that you are
wearing a pair of clean gloves to avoid contamination.
Cheek cells can be
easily obtained by gently scraping the inside of the mouth using a clean,
sterile cotton swab.
Once the cells have been obtained,
the following procedure is used for cheek cell wet mount preparation;
- place a drop of physiological saline on a clean microscopic slide
(central part of the slide)
- smear the cotton swab on to the center (part containing the saline
drop) of the clean slide for about 4 seconds to get the cells on to the center
of the slide
- add a drop of methylene blue solution on to the smear and gently
place a cover slip on top (to cover the stain and the cells)
- any excess solution can be removed by touching one side of the
slide with a paper towel or blotting paper.
- place the slide on the microscope for observation using 4 x or 10
x objective to find the cells
- once the cells have been found, they can then be viewed at higher
*Note- Used cotton swabs
and cotton towel should be safely discarded in the trash and not left lying on
the working table.
Why do we have to Stain the Cells?
The cell has different
parts, and those that can absorb stains or dyes are referred to as chromatic.
Having absorbed the stain, these parts of the cell become more visible under
the microscope and can therefore be easily distinguished from other parts of
the same cell.
Without stains, cells would appear to be almost transparent,
making it difficult to differentiate its parts.
Methylene blue has a string
affinity for both DNA and RNA. When it comes in contact with the two, a darker
stain is produced and can be viewed under the microscope.
The nucleus at the
central part of the cheek cell contains DNA. When a drop of methylene blue is
introduced, the nucleus is stained, which makes it stand out and be clearly
seen under the microscope.
Although the entire cell appears light blue in
color, the nucleus at the central part of the cell is much darker, which allows
it to be identified.
On mounting the wet slide,
the following will be observed;
- Large irregularly shaped cells with distinct cell walls.
- A distinct nucleus at the central part of each individual cell
(dark blue in color).
- A lightly stained cytoplasm in each cell.
This is an easy and fun
experiment that will show kids the basic structure of a cell and its major
parts. For easy identification of the parts, the parent or teacher can first
show the kids some samples of the cells in advance.This will help them
identify different parts with ease.
Once this has been achieved, kids can move
on to the next stage of learning the functions of these different parts.
** Find prepared microscope slides and equipment to correctly perform microscope experiments check out Amazon below.
Other similar fun experiments - Onion Cells , Sugar Crystals, Taking a look at leaves and Hair Under the Microscope
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