The bulb of an onion is formed from modified leaves. While photosynthesis takes place in the leaves of an onion containing chloroplast, the little glucose that is produced from this process is converted in to starch (starch granules) and stored in the bulb.
An onion is made up of layers that are separated by a thin membrane. For this experiment, the thin membrane will be used to observe the onion cells. It can easily be obtained by peeling it from any layer of the onion using tweezers.
**Note- In microscopy, wet mount refers to a glass slide holding a specimen suspended on a drop of liquid for examination.
Moreover, to avoid breaking the slide and damage to the microscope objective lenses during observation, it's important that the optical tube be lowered to the point that the objective lens is as close to the slide as possible.
This should be done starting with low power while looking from the side of the microscope rather than through the eye piece. From this point, it becomes easier to focus for clarity without any accidents.
Students can make another slide without adding the stain to see the difference between a stained slide and a non-stained slide.
The layers of an onion contain simple sugars (carbohydrates) some of which are stored as starch (starch granules). Given that iodine tends to bind to starch, it stains the starch granules when the two come in to contact making them visible.
Although onions may not have as much starch as potato and other plants, the stain (iodine) allows for the little starch molecules to be visible under the microscope. Although onions are plants, students will not see any chloroplasts in their slides.
This is because of the fact that the chloroplast necessary for photosynthesis is largely present in the leafy part of the onion, which is exposed to the sun and absent in the bulb which is below ground and away from sunlight.
Unlike animal cells, students will also notice that the plant cells have a more regular shape. This is because they have a cell wall made up of cellulose which maintains its shape.
This is an easy and fun activity that will allow the student to learn about the cell structure. Students have the opportunity to experiment and observe the onion membrane with different stains (methylene blue and iodine) and without the stain and observe the differences.
Having observed the onion cell under the microscope, students will be able to learn the differences between animal and plant cells in addition to the function of the different parts of the cell.